【病毒外文文獻】2016 Molecular epidemiology of circulating human coronaviruses in children at a tertiary hospital in Catalonia (Spain) f

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S124 Abstracts Journal of Clinical Virology 82S 2016 S1 S142 Conclusion HBoV1 DNA is frequently found in NPAs from chil dren with LRTI in Latvia Although very often HBoV1 infection is accompanied by co infections with other respiratory viruses however there are LRTI cases when HBoV1 is the only pathogen detected indicating its possible role in etiology of the disease http dx doi org 10 1016 j jcv 2016 08 247 Abstract no 316 Presentation at ESCV 2016 Poster 208 Molecular epidemiology of circulating human coronaviruses in children at a tertiary hospital in Catalonia Spain from 2014 to 2016 Javier Ram n Jorgina Vila Cristina Andr s Cintia Castillo Laura Gimferrer Mar a Pi nana Mar a Gema Codina Francisco Fuentes Mar a del Carmen Mart n Rosario Saiz Pilar Alcubilla Carlos Rodrigo Tom s Pumarola Andr s Ant n Hospital Universitari Vall d Hebron Vall d Hebron Research Institute Universitat Aut noma de Barcelona Barcelona Spain Background Human Coronaviruses HCoVs are single stranded positive sense RNA viruses Four HCoVs species 229E OC43 NL63 and HKU1 are currently associated with asymptomatic or mild upper respiratory tract infections URTI in general popu lation but severe acute respiratory infection SARI may occur in patients with high risk of infection such as immunocompromised patients The main aim of this study was to describe the seasona lity and genetic diversity of HCoVs and the clinical features related to HCoVs infection in paediatric patients attended in our hospital from 2014 to 2016 Methods From October 2014 week 40 to May 2016 week 20 respiratory specimens were collected from paediatric patients who were attended at the emergency care unit outpatient departments or admitted to Hospital Universitari Vall d Hebron Barcelona Spain for diagnosis of respiratory viruses by Anyplex II RV16 Detection Kit Seegene Korea that is only able to detect HCoV 229E HCoV OC43 and HCoV NL63 in addition to other respiratory viruses Partial RNA dependent RNA polymerase gene RdRp was sequenced from laboratory con rmed HCoVs specimens for sub sequent phylogenetic analysis in order to con rm the routine diagnostic PCR results In addition partial coding sequence of the spike S glycoprotein was sequenced to identify the different HCoV genotypes Clinical and epidemiological features of HCoV infected cases were retrospectively reviewed from medical records Results A total of 6661 specimens from 3900 patients were received at our laboratory of which 117 2 from 96 patients were positive for HCoVs 11 for HCoV 229E 12 33 for HCoV NL63 34 and 52 for HCoV OC43 54 But phylogenetic analysis of 61 par tial RdRp sequences revealed that viruses were belonging to the four species 6 HCoV 229E 9 15 HCoV NL63 25 22 HCoV OC43 36 and 18 HCoV HKU1 30 HCoVs circulated throughout the year but highest number of detections were shown in autumn months Based on phylogenetic analysis of 69 S sequences HCoV NL63 32 fell into two clusters 16 A 50 16 B 50 HCoV OC43 sequences 19 in two clusters 5 B 26 14 C 74 and HCoV HKU1 18 mainly in other two 16 A 90 1 B 5 but one 5 out of known genetic subgroups HCoV was more often found in respiratory samples of children with URTI 58 had URTI of which 21 were associated with lower respiratory tract infection LRTI 20 5 of patients had LRTI with out URTI and 21 5 were asymptomatic HCoV HKU1 20 and HCoV OC43 29 URTIs were less associated with LRTI than HCoV 229E 50 and HCoV NL63 40 Most of children admitted with HCoV LRTI required supplemental oxygen 11 out of 17 hospitalised patients but only 2 required it for more than 4 days HCoV 229E was related with more oxygen requirements and HCoV OC43 with longer hospitalization stays Only one case was admitted to Paedi atric Intensive Care Unit No fatal cases due to HCoV infection were reported Conclusions Simultaneous circulation of the several HCoVs species was shown from 2014 to 2016 Phylogenetic analysis revealed the circulation of viruses belonging to different genetic subgroups Despite seasonal infection by these four HCoV species is usually related to mild respiratory disease little differences in the clinical features per specie were shown Virological surveil lance must be done to detect changes on the virological and clinical features related to circulating viruses http dx doi org 10 1016 j jcv 2016 08 248 Abstract no 319 Presentation at ESCV 2016 Poster 209 No substantial circulation of enterovirus D68 in patients with severe respiratory disease in South eastern Spain Valencian Community during the 2015 2016 in uenza season Laura Cano 1 Joan Puig Barber 2 3 Javier D ez 2 F Xavier L pez Labrador 1 4 for the Valencia Hospital Network for the Study of In uenza and Respiratory Viruses Disease 4 1 Virology Laboratory Genomics and Health Area Fundaci n para el Fomento de la Investigaci n Sanitaria y Biom dica de la Comunitat Valenciana FISABIO Public Health Valencia Spain 2 Vaccines Research Area Fundaci n para el Fomento de la Investigaci n Sanitaria y Biom dica de la Comunitat Valenciana FISABIO Public Health Valencia Spain 3 Centro de Salud P blica de Castell n Castell n Spain 4 Consorcio de Investigaci n Biom dica de Epidemiolog a y Salud P blic Valencia Spain Background Enterovirus D68 EV D68 was associated with severe respiratory disease in North America and other geographical regions during the fall of 2014 Methods We compared the detection rates of EV D68 in the 2014 2015 in uenza season with that of the 2015 2016 sea son in samples collected in a prospective surveillance scheme for all hospitalizations due to respiratory disease in our region Valencian Community South eastern Spain Combined nasopha ryngeal and nasal children 14 yr old or nasopharyngeal and pharyngeal swabs are analyzed in a single laboratory at FISABIO Public Health for 16 respiratory viruses by multiplex real time RT PCR including rhinovirus enterovirus as a single target All samples positive for rhinovirus enterovirus were retested with a rhinovirus enterovirus discriminative real time RT PCR and those enterovirus positive for EV D68 speci c detection as a single target Results In the 2014 2015 season between November 15th and March 31st 372 of 4472 8 32 samples were rhino enterovirus positive of which 66 17 75 were identi ed as enterovirus and 15 4 03 con rmed as EV D68 In the 2015 2016 season between November 15th and April 30th 201 of 2700 7 45 samples were rhino enterovirus positive of which 42 20 82 were identi ed as enterovirus and only one 0 50 con rmed as EV D68